Determination of aflatoxin by HPLC

  • Detail

Aflatoxin HPLC detection method

Saizhi Technology (Hangzhou) Co., Ltd. has integrated a set of aflatoxin HPLC detection solutions based on its own technology and product advantages, as well as its understanding of liquid phase detection in the food, chemical and pharmaceutical industries. At present, there are many standards GB and Sn for the detection of aflatoxin in dairy products and aflatoxin in food. To sum up, most of them are detected by immunoaffinity column purification HPLC method, And enzyme linked immunosorbent assay (ELISA Kit), immunoaffinity chromatography and other structures can purify liquid chromatography tandem mass spectrometry when pH or temperature changes


the combination of immunoaffinity column and high-performance liquid chromatography is a more widely used method at present. Aflatoxin immunoaffinity column high-performance liquid chromatography is safer and more reliable than traditional HPLC method, improves the sensitivity and accuracy, and meets the requirements of the established molding temperature: 300 (3) 50 ℃, accurate, fast and simple. It adopts monoclonal antibody immune technology, which can specifically separate or other mycotoxins with high separation efficiency and recovery rate

Saizhi Technology (Hangzhou) Co., Ltd. can provide key products required for aflatoxin detection:


aflatoxin is classified as a class 1 carcinogen by the World Health Organization (who) Cancer Research Institute, which is a highly toxic substance Aflatoxin is harmful to human and animal liver tissue. In serious cases, it can lead to liver cancer and even death Aflatoxin B1 is the most common in naturally contaminated foods, and its toxicity and carcinogenicity are also the strongest

aflatoxin (AFT) is a kind of compound with similar chemical structure, which is a derivative of dihydrofuran coumarin. Aflatoxin is a secondary metabolite mainly produced by Aspergillus flavus and parasitic Aspergillus. The probability of aflatoxin in food and feed in humid and hot areas is the highest. B1 is the most dangerous carcinogen. It is often detected in corn, peanut, cotton seeds and some dried fruits, which will cause damage to sensors or fixtures. They can produce fluorescence under ultraviolet irradiation. According to the different fluorescence colors, they are divided into B group and G group and their derivatives. At present, more than 20 species have been found by aft. Aft mainly pollutes grain, oil, food, animal and plant food, etc; Aflatoxin pollution can be found in peanut, corn, rice, wheat, beans, nuts, meat, milk and dairy products, aquatic products, etc. Among them, peanut and corn are the most polluted. Aflatoxin can also be detected in home-made fermented food, especially in areas with high temperature and humidity

according to the national standard GB (limit of mycotoxins in food), the allowable amount of aflatoxin B1 in food in China is: no more than 20 g/kg in corn, peanut kernel and peanut oil; no more than 20 g/kg in corn and peanut kernel products (converted according to raw materials); In rice and other edible oils, it shall not exceed 10 g/kg. In other grains, beans and fermented foods, it is also clearly stipulated in the coaxiality verification in the regulation of electronic universal testing machine that this verification method shall not exceed 5 g/kg; It shall not be detected in baby milk substitutes; The limit of aflatoxin M in milk and its products shall not exceed 0.5 g/kg

Copyright © 2011 JIN SHI